Consider CellServ as Biotechnology Room Service.
Door-to-Door... Lab-to-Lab
Self Contained Laboratory Experiences in
Cell and Tissue Culture Technology
CellServ Kits:
Practical Student Hands-on experience:
Examining a variety of cell types and experimental conditions used in research protocols
Making permanent slides
Quantitative microscopic examinations and analyses
Convenient - No lengthy instructor preparation times
Inexpensive - Approx. $2.00 per student per kit
Utilized by over 300,000 student-lab units nationwide since it's 1989 inception.
Order what you need for when you need it.
Order one or all four kits. The choice is yours.
Suitable for introductory biology, cytology, cell culture, oncology,and developmental biology courses
Using this kit, each student will be led through a step by step procedure which involves staining, mounting, and observation of normal and transformed (tumor) cells. Each student will observe the morphological differences, as well as the differing growth patterns of these cell types when grown in culture.
The manual provided with this kit describes how the cells are grown in culture, what factors influence the transformation of cells, and the differences in growth behavior between normal and tumor cells in culture. Photographs of these cell types are used to guide the students through the observation steps
Kit includes
Enough materials for 30 students working as individuals
Coverslips with IMR-90, a normal, diploid human cell line which shows the typical fibroblastic morphology.
Coverslips with L929, a transformed mouse cell which shows cells of varying morphology and giant cells.
STAIN #1 and STAIN #2.
Permount (mounting medium).
Printed background and procedural information
Glossary of Terms, References, & Further Reading.
Teacher’s Preparation
Monocular or binocular microscope (10 - 40X objectives)
Forceps, pipettes, microscope slides.
Distilled or deionized water aliquotted in small tubes or in squeeze bottles
Time Requirements
30-45 minutes, including casual observations.
Observation of Heterokaryons Through Cell Fusion
Suitable for introductory biology, genetics,
and immunology courses.
Cell fusion has played an important role in gene mapping and hybridoma formation. This kit contains cells of two different species (mouse L929 cells and human fibroblasts) which have been fused and fixed to coverslips prior to shipping. Following a staining and mounting procedure, each student will observe the fused cells and tabulate the incidence of successful heterokaryon formation (mouse cell with human cell).
The manual discusses the methods by which cells are fused and the practical applications of this technology by biomedical researchers. This kit provides a unique way of introducing somatic cell genetics and hybridoma technology and their applications.
Kit includes
Enough materials for 30 students working as individuals
IMR-90 cells, a normal diploid human fibroblast cell line fixed to coverslips.
L929 cells, a transformed, mouse fibroblast cell line, fixed to coverslips.
Fused L929 and IMR-90 cells fixed to coverslips.
STAIN #1 and STAIN #2 (Dif-Quik, Baxter Scientific)
Permount (mounting medium)
Printed background, procedural information
Glossary of Terms, References, and Further Reading section.
Teacher’s Preparation
Monocular or binocular microscope (10X - 40X objectives)
Forceps, pipettes, microscope slides.
Distilled or deionized water aliquotted in small tubes or in squeeze bottles
Time Requirements
60-80 minutes, including casual observations and calculations.
Suitable for introductory biology, cell biology, cytopathology,
and environmental science courses.
With this kit, each student will assess the various types and levels of abnormalities which may arise in cells following exposure to a cytotoxin. Mouse L929 cells are grown on coverslips in the presence of both low and high levels of colchicine. Each student will stain and mount the cells and observe with a microscope the effects of the toxin on cell morphology. Students compare these aberrant morphologies with those of untreated cells. This exercise provides a practical introduction to the field of in vitro toxicology and its importance in biomedical research and environmental science.
The manual provides insights into the practical applications of this important field of study. The accompanying photographs illustrate some typical examples of cellular aberrations.
Kit includes
Enough materials for 30 students working as individuals
L929 cells, a transformed mouse fibroblast cell line, fixed to Leighton tube coverslips.
L929 cells exposed to 1µg/ml of colchicine and fixed to coverslips
L929 cells exposed to 10µg/ml colchicine and fixed to coverslips
STAIN #1 and STAIN #2 (Diff-Quik - Baxter Scientific).
Permount (mounting medium).
Printed background and procedural information
Glossary of Terms, References, & Further Reading
Teacher’s Preparation
Monocular or binocular microscope (10X - 40X objectives)
Forceps, pipettes, microscope slides.
Distilled or deionized water aliquotted in small tubes or in squeeze bottles
Time Requirements
60-80 minutes, including casual observations and calculations.
Suitable for introductory biology, genetics, cytogenetics, cell biology,
and oncology courses.
Using this kit, each student will be provided the unique opportunity of preparing a chromosome spread using human cells. The excitement generated by the students as they “splat” the cells onto microscope slides and proceed to “hunt” for the ideal chromosome spread with the microscope has generated an equal level of excitement among the hundreds of teachers who use this kit. Because the cells used for this exercise are transformed, the number of chromosomes will vary among the cells. Each student can count the chromosomes and see firsthand the range of aneuploidy.
The manual provides information regarding chromosome structure and the important role of karyotyping in genetic disease diagnosis and analysis.
Kit includes
Enough materials for 30 students working as individuals
15 tubes each containing 1 ml of metaphase-blocked cell suspension, fixed in acetic acid-methanol fixative
Stain #1 and Stain #2
Permount (mounting medium)
Printed background and procedural information
Glossary of Terms, References, & Further Reading
Teacher’s Preparation
Binocular or monocular microscopes - magnification of 400X is required to minimally observe the chromosomes while an oil immersion lens (1000X) will ensure the best possible observations.
Pipettes, microscope slides, coverslips
Staining jars or beakers ( = 250ml) containing room temperature water. Alternatively, the slides used for "splatting" of the chromosomes spreads may be dry and at room temperature.
Time Requirements
40-60 minutes